Scaled‐up production of mammalian neural precursor cell aggregates in computer‐controlled suspension bioreactors

• DOI: 10.1002/bit.20900
• Publication Date: 2006-02-18T00:46:48Z
• Authors: Jane A. Gilbertson; Arindom Sen; Leo A. Behie; Michael S. Kallos
• Keywords: Scale-up; Suspension

Abstract The clinical use of neural precursor cells (NPCs) for the treatment neurological diseases, such as Parkinson's disease and Huntington's disease, requires overcoming scarcity these through controlled expansion. main objective present study was to develop a large‐scale computer‐controlled bioprocess expansion mammalian NPCs in suspension culture by scaling up existing reactor protocols. In order support oxygen demands maximum cell densities achieved, volumetric mass transfer coefficient kept above 1.10/h while scaling‐up from small‐scale 125 mL vessels 500 bioreactors. addition, shear stress at impeller tip maintained between 0.30 0.75 Pa reduce damage cells. resulting achieved viable 1.2 × 10 6 cells/mL batch multiplication ratio 9.1. Moreover, process successfully NPC characteristics observed studies. © 2006 Wiley Periodicals, Inc.