PLGA/hydrogel biopapers as a stackable substrate for printing HUVEC networks via BioLP™

• DOI: 10.1002/bit.23295
• Publication Date: 2011-08-09T18:23:20Z
• Authors: Russell Kirk Pirlo; Peter Wu; Jinny Liu; Bradley Ringeisen
• Keywords: Matrigel; Biofabrication; PLGA

Two major challenges in tissue engineering are mimicking the native cell-cell arrangements of tissues and maintaining viability three-dimension (3D) thicker than 300 µm. Cell printing prevascularization engineered promising approaches to meet these challenges. However, technologies used biofabrication must balance competing parameters resolution, speed, volume, which limit resolution 3D structures. We suggest that high-resolution conformal techniques can be print 2D patterns vascular cells onto biopaper substrates then stacked form a construct. Towards this end we created 1 cm × µm biopapers as transferable, stackable substrate for cell printing. 3.6% w/v poly-lactide-co-glycolide was dissolved chloroform poured into molds filled with NaCl crystals. The salt removed DI water scaffolds were dried loaded Collagen Type I or Matrigel. SEM showed extensive porosity gel loading throughout. Biological laser (BioLP) deposit human umbilical vein endothelial (HUVEC) simple intersecting pattern surface biopapers. differentiated stretched networks preserving printed pattern. In separate experiment demonstrate "stackability," individual randomly seeded HUVECs cultured day. mechanically stable viable 4 days. Three-dimensional confocal microscopy infiltration survival compound multilayer constructs. These results feasibility "biopapers" scaffold build vascularized cell-printing technique.