Genomic and metabolic analysis of Komagataeibacter xylinus DSM 2325 producing bacterial cellulose nanofiber

• DOI: 10.1002/bit.27150
• Publication Date: 2019-08-21T09:19:15Z
• Authors: Woo Dae Jang; Tae Yong Kim; Hyun Uk Kim; Woo Yong Shim; Jae Yong Ryu; Jin Hwan Park; Sang Yup Lee
• Keywords: Corynebacterium glutamicum; Metabolic Engineering; Strain (injury); Bacterial Cellulose

Abstract Bacterial cellulose nanofiber (CNF) is a polymer with wide range of potential industrial applications. Several Komagataeibacter species, including xylinus as model organism, produce CNF. However, the application CNF has been hampered by inefficient production, necessitating metabolic engineering for enhanced production. Here, we present complete genome sequence and genome‐scale KxyMBEL1810 K. DSM 2325 Genome analysis this bacterium revealed that set genes associated biosynthesis regulation were in bacterium, which also conserved another six representative species having information. To better understand characteristics 2325, was reconstructed using annotation data, relevant computational resources experimental growth data generated study. Random sampling correlation predicted pgi gnd novel overexpression targets Among engineered strains individually overexpressing heterologous genes, either from Escherichia coli or Corynebacterium glutamicum , batch fermentation strain E. gene produced 3.15 g/L complex medium containing glucose, best concentration achieved study, 115.8% higher than (1.46 g/L) obtained control strain. study should be useful