Analysis of in vitro oxidized human LDL phospholipids by solid‐phase extraction and micellar electrokinetic capillary chromatography

• DOI: 10.1002/bmc.1684
• Publication Date: 2011-08-31T21:35:10Z
• Authors: Ying‐Ling Yang; Chin‐Pong Chong; Ming‐Hua Tsai; Mine‐Yine Liu
• Keywords: Micellar electrokinetic chromatography; Solid phase extraction

ABSTRACT Phospholipids of in vitro oxidized human low‐density lipoproteins (LDL) were separated by two different solid‐phase extraction (SPE) methods. One the methods was designed to test effects gradient elution. This SPE method isolated more phospholipids from LDL than other one according results liquid chromatography and electrospray ionization mass spectrometry (LC ESI‐MS) analysis. A micellar electrokinetic capillary (MEKC) also used analyze SPE. The MEKC LC ESI‐MS consistent for major phospholipid classes, including PC, lysoPC, PE, PI PS. profiles showed significant differences native phospholipids. Therefore, unique combination dramatic distinctions between shows great potential rapid analysis vivo future. Copyright © 2011 John Wiley & Sons, Ltd.