Development and validation of an LC–MS/MS Method for the quantitation of heparan sulfate in human urine

• DOI: 10.1002/bmc.4294
• Publication Date: 2018-05-24T11:04:26Z
• Authors: Kai Wang; Ming Li; Yijin Xiao; Mark Ma; Wei Hu; Tao Liang; Zhongping John Lin
• Keywords: Disaccharide

Heparan sulfate is a linear polysaccharide and serves as an important biomarker to monitor patient response therapies for MPS III disorder. It challenging analyze heparan intact owing its complexity heterogeneity. Therefore, sensitive, robust validated LC-MS/MS method needed support the clinical studies quantitation of in biofluids under regulated settings. Presented this work are results development validation human urine using selected high-abundant disaccharides surrogates. During sample processing, combination analytical technologies have been employed, including rapid digestion, filtration, solid-phase extraction chemical derivatization. The highly sensitive able samples from healthy donors. Disaccharide constitution analysis 25 donors was performed assay demonstrated proof concept surrogate quantitation.