Quantitative analysis of amino acids by HPLC in dried blood and urine in the neonatal period: Establishment of reference values

• DOI: 10.1002/bmc.4931
• Publication Date: 2020-06-26T03:45:23Z
• Authors: Daniela M. Nolasco; Isabel C. P. Fortes; Eugênia R. Valadares
• Keywords: Repeatability; Filter paper; Quantitative Analysis; Dried blood; Sample Preparation; Amino Acid Analysis

Abstract Quantitative analysis of amino acids in blood and urine is primarily indicated for the diagnosis acid disorders. The high‐performance liquid chromatography (HPLC) technique frequently used this detection. frequency sample collection on filter paper has been increasing exponentially, there are many advantages attributed to processing biological samples way. aim study was validate a quantitative by HPLC collected establish reference values neonatal period. Dried dried respectively 58 45 healthy newborns (2–9 days) were collected. Pre‐treatment extraction done according literature. Separation carried out with fluorescence developed method demonstrated excellent separation, linearity, limits detection quantification, repeatability recovery. 17 defined newborns. This work presents simple, fast effective simultaneous single run. established validated.