Screening for α‐glucosidase inhibitors from Selaginella uncinata based on the ligand fishing combined with ultra‐high‐performance liquid chromatography–quadrupole time‐of‐flight–tandem mass spectrometry

• DOI: 10.1002/bmc.5611
• Publication Date: 2023-02-25T08:58:47Z
• Authors: Zhiyu Fan; Gangqiang Yang; Xinjie Wu; Yanan Yang; Jinfang Xu
• Keywords: Biflavonoids; Amentoflavone

Biflavonoids are naturally occurring compounds consisting of two flavonoid moieties that have received substantial attention from researchers. Although many kinds biflavonoids typically distributed in Selaginella uncinata with hypoglycemic effect, their anti-α-glucosidase activities not yet clear. In this study, a ligand fishing strategy for fast screening α-glucosidase inhibitors S. was proposed. α-Glucosidase first immobilized on Fe3 O4 magnetic nanoparticles (MNPs) and then the α-glucosidase-functionalized MNPs were incubated crude extracts to fish out ligands. Furthermore, considering similarity easy confusion structures biflavonoids, fragmentation patterns different types studied. Based this, 11 ligands inhibitory accurately quickly identified ultra-high-performance liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry. these confirmed be potential through vitro assay molecular docking.