A novel cytoprotective organ perfusion platform for reconstructing homeostasis of DCD liver while alleviating IRI injury

• DOI: 10.1002/btm2.10724
• Publication Date: 2024-09-23T09:39:46Z
• Authors: Tingting Lan; Mingxing Yu; Tao Ming; Hong Wang; Juan Deng; Shuhan Cheng; Zhongyang Shen; Deling Kong
• Keywords: Machine Perfusion; Homeostasis

Abstract Pump is a vital component for expelling the perfusate in small animal isolated organ normothermic machine perfusion (NMP) systems whose flexible structure and rhythmic contraction play crucial role maintaining system homeostasis. However, continuous extrusion forming with rigid stationary shaft of peristaltic pumps can damage cells, leading to metabolic disorders eventual dysfunction transplanted organs. Here, we developed novel biomimetic blood‐gas (BBGs) preventing cell damage. This mimics cardiac cycle features an adjustable inspiratory‐to‐expiratory (IE) ratio mitigate acidosis caused by oxygen inhalation. In our study, adipose stem cells (ADSCs) were cultured within circulatory 10 min, 2, 4 h. Compared pump, BBGs significantly reduced apoptosis morphological injury while enhancing proliferation adhesion. Additionally, when supernatant from ADSCs was introduced LPS‐induced macrophages 24 h, group demonstrated more pronounced anti‐inflammatory effect, characterized M1 macrophage expression. Besides, rat livers donation after death (DCD) 6 h BBGs, detected fewer apoptotic inflammatory response, evidenced down‐regulated TNF‐α The development demonstrates feasibility recreating physiological liquid–gas circulation vitro, offering alternative platform perfusion, especially applications involving therapy.