Roles for VEGF‐C/NRP–2 axis in regulating renal tubular epithelial cell survival and autophagy during serum deprivation

• DOI: 10.1002/cbf.3402
• Publication Date: 2019-06-18T12:49:34Z
• Authors: Xiaoyan Chang; Qian Yang; Conghui Zhang; Ying Zhang; Xinjun Liang; Yanyan Liu; Gang Xu
• Keywords: Serum; 0301 basic medicine; 0303 health sciences; Cell Survival; Vascular Endothelial Growth Factor C; Epithelial Cells; Cell Line; Neuropilin-2; Rats; 03 medical and health sciences; Kidney Tubules; Autophagy; Animals; Research Articles; Signal Transduction

Vascular endothelial growth factor C (VEGF-C) is an angiogenic and lymphangiogenic factor. Recent research has revealed the role for VEGF-C in regulating autophagy by interacting with a nontyrosine kinase receptor, neuropilin-2 (NRP-2). However, whether participates cell survival renal proximal tubular cells unknown. To address this question, we employed modal of serum deprivation to verify its receptor NRP-2 NRK52E lines. The results show that rescued loss viability induced concentration-dependent manner. Furthermore, endogenous was knocked down using specific small-interfering RNAs (siRNA), were more sensitive deprivation-induced death. A similar increase death rate observed following depletion serum-starved cells. Autophagy activity confirmed western blot analysis microtubule-associated protein-1 chain 3 (LC3), immunofluorescence staining LC3, formation autophagosomes electron microscopy. or further increased LC3 expression deprivation, suggesting involved controlling We performed autophagic flux experiments identify promotes activation Together, these suggest first time VEGF-C/NRP-2 axis under condition. SIGNIFICANCE OF THE STUDY: More researchers had focused on regulation kidney disease. effect epithelial not been examined. identified as regulator And may mediate phosphorylation 4EBP1 P70S6K. treatment be therapeutic target injury repair due capacity promote future.