Probing Lectin and Sperm with Carbohydrate‐Modified Quantum Dots

• DOI: 10.1002/cbic.200500112
• Publication Date: 2005-09-08T16:09:14Z
• Authors: Anandakathir Robi...; Jim‐Min Fang; Pi‐Tai Chou; Kuang‐Wen Liao; Rea‐Min Chu; Shyh‐Jye Lee
• Keywords: Peanut agglutinin

Abstract We report the encapsulation of quantum dots with biologically important β‐ N ‐acetylglucosamine (GlcNAc) in different ratios, together studies their specific/sensitive multivalent interactions lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering confocal imaging techniques, flow cytometry. These GlcNAc‐encapsulated (QDGLNs) specifically bind to wheat germ agglutinin, cause fluorescence quenching aggregation. Further QDGLNs mannose‐encapsulated QDs (QDMANs) revealed site‐specific interactions, which head sperm, while QDMANs spread over whole body.