Luminescent Conjugated Oligothiophenes for Sensitive Fluorescent Assignment of Protein Inclusion Bodies

• DOI: 10.1002/cbic.201200731
• Publication Date: 2013-03-01T02:04:36Z
• Authors: Therése Klingstedt; Cristiane Blechsc...; Anna Nogalska; Stefan Prokop; Bo Häggqvist; Olof Danielsson; W. King Engel; Valerie Askanas; Frank L. Heppner; K. Peter R. Nilsson
• Keywords: Fluorescent protein

Abstract Small hydrophobic ligands identifying intracellular protein deposits are of great interest, as inclusion bodies the pathological hallmark several degenerative diseases. Here we report that fluorescent amyloid ligands, termed luminescent conjugated oligothiophenes (LCOs), rapidly and with high sensitivity detect in skeletal muscle tissue from patients sporadic body myositis (s‐IBM). LCOs having a backbone at least five thiophene units emitted strong fluorescence upon binding, showed co‐localization proteins reported to accumulate s‐IBM bodies. Compared conventional identified larger fraction immunopositive When was extended terminal carboxyl groups, LCO revealed striking spectral differences between distinct We conclude 1) sensitive, rapid powerful tools for 2) identify wider range than ligands.