Design and Synthesis of 6‐O‐Phosphorylated Heparan Sulfate Oligosaccharides to Inhibit Amyloid β Aggregation

• DOI: 10.1002/cbic.202200191
• Publication Date: 2022-05-19T05:50:12Z
• Authors: Kenji Uchimura; Kazuchika Nishitsuji; Li‐Ting Chiu; Takashi Ohgita; Hiroyuki Saito; Fabrice Allain; Veeranjaneyulu Ga...; Chi‐Huey Wong; Shang‐Cheng Hung
• Keywords: Thioflavin; Amyloid (mycology)

Dysregulation of amyloidogenic proteins and their abnormal processing deposition in tissues cause systemic localized amyloidosis. Formation amyloid β (Aβ) fibrils that deposit as plaques Alzheimer's disease (AD) brains is an earliest pathological hallmark. The polysulfated heparan sulfate (HS)/heparin (HP) one the non-protein components Aβ deposits not only modulates aggregation, but also acts a receptor for to mediate cytotoxicity. Interfering with interaction between HS/HP could be therapeutic strategy arrest Here we have synthesized 6-O-phosphorylated oligosaccharides reported competitive effects on inhibition HP-mediated fibril formation vitro using thioflavin T fluorescence assay tapping mode atomic force microscopy.