MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

• DOI: 10.1002/cbin.10704
• Publication Date: 2016-11-15T00:18:56Z
• Authors: Lu Liu; Mengjun Liu; Rongrong Li; Hong Liu; Liling Du; Hong Chen; Yan Zhang; Shijie Zhang; Dongxu Liu
• Keywords: Bone Formation

Cyclical stretch-induced bone formation during orthodontic treatment is a complex biological process modulated by various factors including miRNAs and their targeted-gene network. However, the miRNA expression profile roles in osteogenic differentiation of mesenchymal stem cells (BMSCs) exposed to mechanical stretch remains unclear. Here, we use microarray assay screen for mechano-sensitive BMSCs identified that nine were differentially expressed between stretched control BMSCs. Furthermore, miR-503-5p, which was markedly downregulated both qRT-PCR selected further functional verification. We found overexpression miR-503-5p attenuated while effect reversed inhibition treatment. In vivo studies, with specific agomir decreased Runx2, ALP mRNA, protein expression, osteoblast numbers osteoblastic OTM tension sides. conclusion, our study revealed functions as inhibits subjected