Caveolae Modulate the Activity of LRRC8‐Mediated VRAC by the Structural Membrane Protein Caveolin‐1

• DOI: 10.1002/cbin.70001
• Publication Date: 2025-02-15T13:00:01Z
• Authors: Yan Liu; Xing Li; Cong Huo; Liming Hou; Xin Jia; Rong Xu; Jie Yang; Xiaoming Wang
• Keywords: Caveolin 1; Caveolin; Lipid raft; Immunoprecipitation

ABSTRACT The volume‐regulated anion channel (VRAC) plays a critical role in cell volume regulation and other fundamental physiological processes. However, the mechanism of how VRAC is activated modulated has not been completely clarified. Caveolin‐1 (Cav‐1), as an important ion binding protein, forms complexes with proteins exchangers to regulate activity function. purpose this study was explore importance value Cav‐1 cardiac activation regulation. In study, we proved that membrane protein LRRC8A detected same caveolae‐enriched fractions, ventricular myocytes. intracellular Cl − concentration increased decreased dramatically after caveolae being destroyed cardiomyocytes. Moreover, found I Cl,vol only silencing cardiomyocytes but also cardiomyocytes, which indicated caveolin‐1 may affect function VRAC. Then further physical relationship between membrane. We observed fluorescence label overlapping plasma co‐immunoprecipitated LRRC8A, demonstrated basis by acting on LRRC8A. whole provides evidence relevance modulation endothelial LRRC8A‐mediated