Biofuel‐Cell Cathodes Based on Bilirubin Oxidase Immobilized through Organic Linkers on 3D Hierarchically Structured Carbon Electrodes

• DOI: 10.1002/celc.201402099
• Publication Date: 2014-08-11T20:14:44Z
• Authors: Jeevanthi Vivekan...; Rosalba A. Rincón; Volodymyr Kuznetsov; Sascha Pöller; Wolfgang Schuhmann
• Keywords: Bilirubin oxidase; Glucose oxidase; Carbon fibers

Abstract Different modification procedures to stabilize and control the orientation of Myrothecium verrucaria bilirubin oxidase ( Mv BOD) on 3D carbon nanotube/carbon microfiber‐modified graphite electrode surfaces were evaluated for development biofuel‐cell cathodes. The surface properties different linkers covalent binding BOD investigated by using atomic force microscopy‐based techniques. For all immobilization strategies, maximal current response was obtained at a pH value 6.5 with temperatures between 20 35 °C. biocathode based immobilized through an imino bond showed highest density (1600 μA cm −2 ) resistant presence chloride ions. A biofuel cell constructed, it exhibited power 54 μW 350 mV open‐circuit voltage about 600 cellobiose dehydrogenase bioanode glucose as fuel.