Physicochemical Characterization of Sparsely Tethered Bilayer Lipid Membranes: Structure of Submembrane Water and Nanomechanical Properties

• DOI: 10.1002/celc.202100721
• Publication Date: 2021-07-09T03:37:35Z
• Authors: Damian Dziubak; Slawomir Sek
• Keywords: Characterization

Abstract Sparsely tethered bilayer lipid membrane (stBLM) is a popular model considered to mimic biological membranes. The advantage of this system the molecular architecture that provides water environment on both sides membrane. In work, we have coupled electrochemical measurements with surface enhanced infrared absorption spectroscopy and atomic force microscopy evaluate effect electric field physicochemical properties stBLM composed 1,2‐dimyristoyl‐ sn ‐glycero‐3‐phosphocholine cholesterol. More specifically, focused structure submembrane nanomechanical Our experimental results revealed DMPC/Cholesterol offers good stability low permeability within relatively wide potential window. Importantly, it also covers biologically relevant range corresponding transmembrane potentials occurring in natural cell membranes, which makes them suitable platform for bioinspired sensing devices.