Circular dichroism detection in HPLC

• DOI: 10.1002/chir.530030424
• Publication Date: 2005-01-07T11:11:46Z
• Authors: Piero Salvadori; Carlo Bertucci; Carlo Rosini

AbstractA circular dichroism‐based detection system presents several advantages in the HPLC analysis of chiral compounds because of the selective monitoring of optically active molecules. Its use allows reliable determination of enantiomeric excesses and elution order. To this end, the application of empirical, semiempirical, and nonempirical methods to get stereochemical information from the CD signal is reported. Furthermore, recording the CD spectra on line and evaluation of the dissymetry factor make the CD detection very powerful in characterizing the stereochemistry of chiral eluates.