Serum Circulating mRNA Panel for the Early Detection of Gastric Cancer: A Potential Biomarker Test

• DOI: 10.1002/cmdc.202400523
• Publication Date: 2024-09-05T11:00:44Z
• Authors: Xinyu Peng; Xiaoyan Teng; Qian Ma; Da Han
• Keywords: Diagnostic biomarker

Circulating free messenger RNAs (cfmRNAs) in serum have emerged as potential noninvasive biomarkers for cancer diagnosis, including gastric (GC). This study utilized RNA‐sequencing data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases to identify a training set of 100 differentially expressed genes (DEGs) specific GC patients. Employing support vector machine (SVM) classification, we narrowed down the candidate gene 23, which was further refined 4 genes—DMBX1, EVX1, MAL, PIWIL1—after validation through reverse transcription quantitative polymerase chain reaction (RT‐qPCR). diagnostic performance mRNA panels, particularly combinations DMBX1 with EVX1 PIWIL1, exceptional, achieving area under curve (AUC) values 0.800, sensitivities 90.0%, specificities 80.0%. accuracy these corroborated various learning algorithms, underscoring their robust potential. findings this are poised significantly influence clinical practice by providing tools early detection. As undergo investigation validation, they hold promise become integral GC.