Distribution of aromatase mRNA and protein in the brain and pituitary of female rainbow trout: Comparison with estrogen receptor α

• DOI: 10.1002/cne.10726
• Publication Date: 2003-06-03T10:08:03Z
• Authors: Arnaud Menuet; Isabelle Anglade; Remy Le Guevel; Elisabeth Pellegrini; Farzad Pakdel; Olivier Kah
• Keywords: Preoptic area; Diencephalon

Abstract Recent data indicate that estrogens locally produced in the brain by aromatization of androgens could be important for neurogenesis and repair. In this respect, fish are interesting because extremely high aromatase activity their brain. study, rainbow trout was cloned riboprobes were used to map distribution cells expressing corresponding mRNAs. A very strong hybridization signal detected pituitary bordering ventricles telencephalon ventral diencephalon, with highest expression preoptic area hypothalamus. weaker ependymal layer torus semicircularis optic tectum. This localization fully confirmed immunohistochemistry using antibodies against a teleost aromatase. addition, antibody showed fact corresponds radial glial immunoreactive had long cytoplasmic processes extending toward pial surface. Because shown upregulated estradiol fish, mRNAs compared estrogen receptor α (rtERα) on adjacent sections. Although found regions rtERα, no obvious coexpression found, as rtERα never observed cells. However, reverse transcriptase‐polymerase chain reaction experiments performed cell cultures enriched suggest weak cannot excluded. The possible role content related continuous growth central nervous system during adulthood. J. Comp. Neurol. 462:180–193, 2003. © 2003 Wiley‐Liss, Inc.