Optimized NMR Experiments for the Isolation of I=1/2 Manifold Transitions in Methyl Groups of Proteins

• DOI: 10.1002/cphc.201900959
• Publication Date: 2019-11-08T20:10:25Z
• Authors: Vitali Tugarinov; Theodoros K. Kara...; Alberto Ceccon; G. Marius Clore
• Keywords: Ubiquitin; Malate Synthase; Humans; Nuclear Magnetic Resonance, Biomolecular; 01 natural sciences; 0104 chemical sciences

Abstract Optimized NMR experiments are developed for isolating magnetization belonging to the I =1/2 manifolds of 13 CH 3 methyl groups in proteins, enabling manipulation a moiety as if it were an AX ( 1 H‐ C) spin‐system. These result same ‘simplification’ spin‐system that would be obtained from production { CHD 2 }‐methyl‐labeled protein samples. The sensitivity manifold‐selection is factor approximately less than corresponding acquired on }‐labeled groups. methodology described here primarily intended small‐to‐medium sized where losses associated with isolation manifold transitions can tolerated. Several applications benefit simplification (AX ) spin‐systems described, emphasis measurements C residual dipolar couplings deletion mutant human chaperone DNAJB6b, modulation signal intensities due evolution scalar and follows simple cosine function characteristic spin‐system, significantly simplifying data analysis.