Cytomegalovirus Infection: Mouse Model

• DOI: 10.1002/cpim.51
• Publication Date: 2018-07-25T14:59:29Z
• Authors: Ilija Brizić; Berislav Lisnić; Wolfram Brune; Hartmut Hengel; Stipan Jonjić
• Keywords: 0303 health sciences; disease models; mice; BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences.; viral plaque assay; 610; salivary gland; herpesviridae infections; mouse cytomegalovirus; cytomegalovirus infections; viral load; virology; 3. Good health; 03 medical and health sciences; fibroblasts; mice ; cytomegalovirus infections/virology ; disease models ; animal ; virus replication ; viral plaque assay/methods ; viral load ; salivary gland/virology ; mouse cytomegalovirus/isolation & purification ; herpesviridae infections/virology ; fibroblasts/virology ; MCMV; BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti.; MCMV; virus replication

AbstractThis unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay. © 2018 by John Wiley & Sons, Inc.