Whole blood transcriptomics reveals granulocyte colony‐stimulating factor as a mediator of cardiopulmonary bypass‐induced systemic inflammatory response syndrome
0301 basic medicine
0303 health sciences
610
whole blood transcriptomics
RC581-607
cytokines
granulocyte colony‐stimulating factor
systemic inflammatory response syndrome
Original Article
Immunologic diseases. Allergy
cardiopulmonary bypass
cell‐free DNA
DOI:
10.1002/cti2.1490
Publication Date:
2024-02-19T08:16:25Z
AUTHORS (9)
ABSTRACT
Abstract Objectives Systemic inflammatory response syndrome (SIRS) is a frequent complication of cardiopulmonary bypass (CPB). SIRS associated with significant morbidity and mortality, but its pathogenesis remains incompletely understood, as result, biomarkers are lacking treatment expectant supportive. This study aimed to understand the pathophysiological mechanisms driving induced by CPB identify novel therapeutic targets that might reduce systemic inflammation improve patient outcomes. Methods Twenty‐one patients undergoing cardiac surgery were recruited, blood was sampled before, during after surgery. defined using American College Chest Physicians/Society Critical Care Medicine criteria. We performed immune cell profiling whole transcriptomics measured individual mediators in plasma/serum characterise CPB. Results Nineteen fulfilled criteria for SIRS, mean duration 2.7 days. Neutrophil numbers rose rapidly remained elevated at least 48 h afterwards. Transcriptional signatures neutrophil activation degranulation enriched identified network cytokines governing these transcriptional changes, including granulocyte colony‐stimulating factor (G‐CSF), regulator production function. Conclusions neutrophils G‐CSF major regulators CPB‐induced inflammation. Short‐term targeting could provide strategy limit neutrophil‐mediated tissue damage
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