Whole blood transcriptomics reveals granulocyte colony‐stimulating factor as a mediator of cardiopulmonary bypass‐induced systemic inflammatory response syndrome

• DOI: 10.1002/cti2.1490
• Publication Date: 2024-02-19T08:16:25Z
• Authors: Katherine R Martin; Cristina Gamell; Tsin Yee Tai; Roberto Bonelli; Jacinta Hansen; James Tatoulis; Monther Alhamdoosh; Nicholas Wilson; Ian Wicks
• Keywords: 0301 basic medicine; 0303 health sciences; 610; whole blood transcriptomics; RC581-607; cytokines; granulocyte colony‐stimulating factor; systemic inflammatory response syndrome; Original Article; Immunologic diseases. Allergy; cardiopulmonary bypass; cell‐free DNA

AbstractObjectivesSystemic inflammatory response syndrome (SIRS) is a frequent complication of cardiopulmonary bypass (CPB). SIRS is associated with significant morbidity and mortality, but its pathogenesis remains incompletely understood, and as a result, biomarkers are lacking and treatment remains expectant and supportive. This study aimed to understand the pathophysiological mechanisms driving SIRS induced by CPB and identify novel therapeutic targets that might reduce systemic inflammation and improve patient outcomes.MethodsTwenty‐one patients undergoing cardiac surgery and CPB were recruited, and blood was sampled before, during and after surgery. SIRS was defined using the American College of Chest Physicians/Society of Critical Care Medicine criteria. We performed immune cell profiling and whole blood transcriptomics and measured individual mediators in plasma/serum to characterise SIRS induced by CPB.ResultsNineteen patients fulfilled criteria for SIRS, with a mean duration of 2.7 days. Neutrophil numbers rose rapidly with CPB and remained elevated for at least 48 h afterwards. Transcriptional signatures associated with neutrophil activation and degranulation were enriched during CPB. We identified a network of cytokines governing these transcriptional changes, including granulocyte colony‐stimulating factor (G‐CSF), a regulator of neutrophil production and function.ConclusionsWe identified neutrophils and G‐CSF as major regulators of CPB‐induced systemic inflammation. Short‐term targeting of G‐CSF could provide a novel therapeutic strategy to limit neutrophil‐mediated inflammation and tissue damage in SIRS induced by CPB.