Histone demethylase JMJD1C promotes the polarization of M1 macrophages to prevent glioma by upregulating miR‐302a

• DOI: 10.1002/ctm2.424
• Publication Date: 2021-09-27T03:32:05Z
• Authors: Chuanhong Zhong; Bei Tao; Feilong Yang; Kaiguo Xia; Xiaobo Yang; Ligang Chen; Tangming Peng; Xiangguo Xia; Xianglong Li; Lilei Peng
• Keywords: Demethylase; Macrophage polarization

Abstract Glioma is regarded as an aggressive lethal primary brain tumor. Jumonji domain containing 1C (JMJD1C) a H3K9 demethylase which participates in the progression of various tumors, but its specific function and underlying mechanism glioma development remain undefined, purpose our work. We initially assessed JMJD1C expression tissues cells using assays RT‐qPCR immunohistochemistry. Meanwhile, level at microRNA (miR)‐302a promoter region was measured by chromatin immunoprecipitation assay, while luciferase‐based reporter assay performed for validation binding affinity between miR‐302a methyltransferase‐like 3 (METTL3). The effect METTL3 on suppressor cytokine signaling 2 (SOCS2) subsequently analyzed MeRIP‐RT‐qPCR. Finally, xenograft tumor model established nude mice, followed measurement tumor‐associated macrophages flow cytometry. poorly expressed tissues. Furthermore, increased through promoting H3K9me1 demethylation region. identified to target METTL3, could inhibit SOCS2 via m6A modification. promoted M1 macrophage polarization suppressed growth xenografts miR‐302a/METTL3/SOCS2 axis both vivo vitro. In conclusion, enhance onset glioma, bringing new insight into contribution pathobiology with possible implications targeted therapeutic method.