Ginsenoside Rg3 attenuates ovariectomy‐induced osteoporosis via AMPK/mTOR signaling pathway

• DOI: 10.1002/ddr.21705
• Publication Date: 2020-06-17T17:10:54Z
• Authors: Xiaonan Zhang; Fenglan Huang; Xiaoyong Chen; Xiaoqing Wu; Jinyu Zhu
• Keywords: Viability assay

Abstract Ginsenoside Rg3, a ginsenoside isolated from Panax ginseng , can regulate autophagy via AMP‐activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signaling pathway. AMPK/mTOR and have been reported to be involved in osteogenesis. Here, the effect Rg3 on ovariectomy (OVX)‐induced osteoporosis is explored. In vivo, rats were treated with 20 mg/kg after OVX body weight (BW) was monitored. Bone mineral density (BMD), hematoxylin–eosin staining femur tissues, osteogenesis, autophagy, analyzed. vitro, MC3T3‐E1 cells 0, 1, 5, 10, 20, 100 μmol/L Rg3. 10 which had no significant cell viability significantly affected signaling, chosen for further analysis. Then osteogenic differentiation induced or/and AMPK inhibitor (Compound C). differentiation, mineralization by Alizarin Red The expression or activity signaling‐related proteins, markers, osteogenesis markers measured western blotting commercial kits, counting kit‐8 assay kits. alleviated OVX‐induced BW increases, BMD declines histological changes promoted but inhibited mTOR vivo. Moreover, enhanced mineralization, suppressed vitro. However, Compound C reversed Rg3‐induced alterations indicating that regulated signaling. Hence, it speculated might attenuate