Maternal glucose intolerance reduces offspring nephron endowment and increases glomerular volume in adult offspring

• DOI: 10.1002/dmrr.2805
• Publication Date: 2016-04-02T23:10:18Z
• Authors: Stacey Hokke; Nicole Arias; James A. Armitage; Victor G. Puelles; Karen Fong; Stefania Geraci; Norbert Gretz; John F. Bertram; Luise A. Cullen‐M...
• Keywords: Mice, Knockout; 0301 basic medicine; Fetal Growth Retardation; Kidney Glomerulus; Maternal Nutritional Physiological Phenomena; Nephrons; Embryo, Mammalian; Weight Gain; Mice, Inbred C57BL; Diabetes, Gestational; Mice; 03 medical and health sciences; Pregnancy; Prenatal Exposure Delayed Effects; Glucose Intolerance; Animals; Receptors, Leptin; Female

Animal studies report a nephron deficit in offspring exposed to maternal diabetes, yet are limited models of severe hyperglycaemia which do not reflect the typical clinical condition and associated with foetal growth restriction that may confound endowment. We aimed assess renal morphology function leptin receptor deficient mice (Leprdb /+) hypothesized exposure impaired glucose tolerance (IGT) would be detrimental developing kidney.Nephron endowment was assessed C57BKS/J Leprdb /+ +/+ at embryonic day (E)18 postnatal (PN)21 using design-based stereology. Transcutaneous measurement total glomerular volume were 6-month-old offspring. Only dams analysed.Compared dams, had 20% 35% decrease prior pregnancy E17.5 respectively. Offspring IGT approximately 15% fewer nephrons E18.5 PN21 than dams. There no difference bodyweight. Despite normal function, 13% greater offspring.IGT throughout gestation resulted established early development. Maternal hypertrophy adult offspring, likely compensatory response maintain function. Given increasing prevalence IGT, monitoring from important prevent altered kidney morphology. Copyright © 2016 John Wiley & Sons, Ltd.