Loss of Lrp2 in zebrafish disrupts pronephric tubular clearance but not forebrain development

• DOI: 10.1002/dvdy.22624
• Publication Date: 2011-03-31T13:09:05Z
• Authors: Esther Kur; Anna Christa; Kerry N. Veth; Chandresh R. Gajera; Miguel A. Andrade...; Jingjing Zhang; Jason R. Willer; Ronald G. Gregg; Salim Abdelilah‐S...; Sebastian Bachmann; Brian A. Link; Annette Hammes; Thomas E. Willnow
• Keywords: Forebrain; Pronephros; Morpholino

Abstract Low‐density lipoprotein receptor‐related protein 2 (LRP2) is a multifunctional cell surface receptor conserved from nematodes to humans. In mammals, it acts as regulator of sonic hedgehog and bone morphogenetic pathways in patterning the embryonic forebrain clearance adult kidney. Little known about activities this LRP other phyla. Here, we extend functional elucidation LRP2 zebrafish model organism (dys)function. We demonstrate that expression Lrp2 larval fish recapitulates patterns seen mammalian brain Furthermore, studied consequence deficiencies lrp2 lrp2b , homologue unique fish, using ENU mutagenesis or morpholino knockdown. While receptor‐deficient suffer overt renal resorption deficiency, their development proceeds normally, suggesting evolutionary conservation functions pronephric duct but not teleost forebrain. Developmental Dynamics 240:1567–1577, 2011. © 2011 Wiley‐Liss, Inc.