Detection of C Reactive Protein (CRP) in Serum by an Electrochemical Aptamer‐Based Sandwich Assay

• DOI: 10.1002/elan.200804560
• Publication Date: 2009-05-04T13:18:00Z
• Authors: Sonia Centi; Laura Bonel Sanma...; Sara Tombelli; Ilaria Palchetti; Marco Mascini
• Keywords: Differential pulse voltammetry; Aptamer; Glassy carbon

Abstract A disposable electrochemical assay involving magnetic particles and carbon‐based screen‐printed electrodes (SPCEs) was developed for the detection of C Reactive Protein (CRP). CRP is a plasma protein among most expressed proteins in acute phase inflammation cases, being known biomarker inflammatory states. The based on sandwich format which RNA aptamer coupled to monoclonal antibody alkaline phosphatase (AP) used as enzymatic label. After assay, modified beads were captured by magnet surface graphite working electrode thus achieved through addition AP substrate (α‐naphthyl‐phosphate) α‐naphthol produced during reaction detected using differential pulse voltammetry (DPV). parameters influencing different steps optimized order reach best sensitivity specificity. With conditions, applied analysis free serum samples.