An updated two‐dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies

• DOI: 10.1002/elps.11501601324
• Publication Date: 2005-05-28T14:29:35Z
• Authors: N. Leigh Anderson; Ricardo Esquer‐Bl...; Jean‐Paul Hofmann; Lydie Meheus; Jos Raymackers; Sandra Steiner; Frank Witzmann; Norman G. Anderson
• Keywords: Spots; Coomassie Brilliant Blue

Abstract We have improved upon the reference two‐dimensional (2‐D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al. , Electrophoresis 1991, 12 907–930). A total 53 (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2‐D gels were submitted to internal tryptic digestion [2], and individual peptides, separated high‐performance liquid chromatography (HPLC), sequenced using a Perkin‐Elmer 477A sequenator. Additional identified specific antibodies.