Equalizer technology followed by DIGE‐based proteomics for detection of cellular proteins in artificial peritoneal dialysis effluents

• DOI: 10.1002/elps.201300499
• Publication Date: 2014-03-04T01:03:10Z
• Authors: Anton Michael Lic...; Rebecca Herzog; Silvia Tarantino; Christoph Aufricht; Klaus Kratochwill
• Keywords: Biomarker Discovery

Peritoneal dialysis effluent (PDE) represents a rich pool of potential biomarkers for monitoring disease and therapy. Until now, proteomic studies have been hindered by the plasma-like composition PDE. Beads covered with peptide library are promising approach to remove high abundant proteins concentrate sample in one step. In this study, novel biomarker identification PDEs consisting depletion concentration step followed 2D gel based protein quantification was established. To prove experimental concept model system artificial established spiking unused peritoneal (PD) fluids cellular reflecting control conditions or cell stress. Using procedure, we were able reduce amount plasma low while preserving changes abundance origin. The alterations investigated marker stress, heat shock proteins, showed similar profiles PDE as pure culture samples. Our results demonstrate efficacy detecting subtle expression triggered unphysiological stress stimuli typical PD, which could serve biomarkers. Further using patients' will be necessary clinical PD assess whether technique is also informative regarding enriching derived