Effects of chitosan on the protein profile of grape cell culture subcellular fractions

• DOI: 10.1002/elps.201300624
• Publication Date: 2014-03-03T09:35:18Z
• Authors: Maura Ferri; Marina Franceschetti; Michael J. Naldrett; Gerhard Saalbach; Annalisa Tassoni
• Keywords: Nutraceutical

Grapevine is a large source of healthy polyphenols for human diet, and red table‐grapes wines are the main stilbenes. These compounds important both in plant defence system health. In present study, Vitis vinifera cv. Barbera cell cultures were treated with 50 μg/mL chitosan proteomic analyses on soluble membrane subcellular fractions performed against suitable controls. Three stilbene synthase protein spots, four spots microsomal fraction ATPase subunits identified, accumulation which was modulated response to treatment. Present immunolocalisation data seem provide evidence supporting hypothesis that biosynthetic multi‐enzyme complex associated intracellular membrane. addition, showed general decrease proteins belonging different primary metabolism pathways, fractions. particular, energy, sugar amino acid metabolisms down‐regulated as consequence acetic treatments. metabolic modifications could lead consistent change profile amount metabolites stored grape berries, consequent effects taste, flavour, organoleptic nutraceutical properties derived food products.