Development of a microchip capillary electrophoresis method for determination of the purity and integrity of mRNA in lipid nanoparticle vaccines

• DOI: 10.1002/elps.202100272
• Publication Date: 2021-11-22T09:56:15Z
• Authors: Jessica Raffaele; John W. Loughney; Richard R. Rustandi
• Keywords: Electrophoresis, Microchip; 0301 basic medicine; Vaccines; 0303 health sciences; 03 medical and health sciences; Liposomes; Electrophoresis, Capillary; Nanoparticles; RNA, Messenger; 3. Good health

Messenger RNA (mRNA)-based vaccines are advantageous because they can be relatively quicker and more cost efficient to manufacture compared other traditional vaccine products. Lipid nanoparticles have three common purposes: delivery, self-adjuvanting properties, mRNA protection. Faster development requires an fast assay monitor purity integrity. Microchip CE is known a robust technology that capable of rapid separation. Here, we describe the optimization integrity for mRNA-based encapsulated in lipid using commercial microchip-based The analytical parameters optimized were assessed method stability indicating assay.