Bifidobacterium bifidum CCFM1163 Alleviates Cathartic Colon Through the Acetate/Propionate‐GPR41‐Gβγ Pathway

• DOI: 10.1002/fft2.526
• Publication Date: 2024-11-26T05:41:46Z
• Authors: Yin Huang; Nan Tang; Yifan Xue; Jiazhen Li; WenXu Liu; Shengnan Zhu; Linlin Wang; Gang Wang; Wei Chen; Jianxin Zhao
• Keywords: Bifidobacterium bifidum; Cathartic

ABSTRACT Cathartic colon, a form of slow‐transit constipation, arises from prolonged stimulant laxative use and is associated with intestinal barrier impairment enteric nervous system damage. We identified Bifidobacterium bifidum CCFM1163 as an effective agent for alleviating cathartic colon; however, the underlying mechanisms remain unclear. This study first administered live, dead, supernatant forms to colon mice, finding that only live bacteria were effective. Non‐targeted targeted metabolomics analyses mouse fecal metabolites highlighted short‐chain fatty acids (SCFAs) key players. Animal experiments comparing effects major SCFAs revealed acetic acid (AA) propionic (PA) ameliorated (reduces transit time, p < 0.0001; increases water content, 0.05). Inhibition studies using receptor inhibitors downstream demonstrated both HAS (a G protein‐coupled 41 [GPR41] inhibitor) Gallein (Gi protein βγ subunit [Gβγ] negated reparative on nerves in mice. was shown modulate flora, notably increasing Faecalibaculum, Candidatus Saccharimonas, Prevotellaceae UCG‐001 ( 0.05) decreasing Escherichia, Clostridioides 0.0001), while elevating AA PA levels AAs act GPR41 receptor, activating Gβγ, thus enhancing reducing tissue inflammation. primarily repairs via GPR41‐Gβγ, promoting peristalsis. elucidates B. CCFM1163's potential relief provides theoretical basis probiotic treatment.