Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangements

• DOI: 10.1002/gcc.20092
• Publication Date: 2004-08-27T21:39:00Z
• Authors: Yuri N. Kobzev; Jose Martinez‐Cli...; Sanggyu Lee; Jianjun Chen; Janet D. Rowley
• Keywords: Breakpoint; Hematology

Abstract The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias 11p15 translocations. We report the results of screening cases cytogenetically documented rearrangements and subsequent identification involvement its genes. identified 49 samples from 46 hematology patients (including a few 11p14) abnormalities, using fluorescence situ hybridization (FISH), we found that was disrupted 7 cases. With use gene‐specific FISH probes, 6 cases, genes, which were PRRX1 ( PMX1; 2 cases), HOXD13 , RAP1GDS1 HOXC13 TOP1 . In 3 for RNA available, RT‐PCR performed, confirmed location breakpoints patient cDNA. Our data confirm previous findings is recurrent target various types leukemia. © 2004 Wiley‐Liss, Inc.