High‐resolution copy number and gene expression microarray analyses of head and neck squamous cell carcinoma cell lines of tongue and larynx

• DOI: 10.1002/gcc.20551
• Publication Date: 2008-03-03T23:16:37Z
• Authors: Anna‐Kaarina Järv...; Reija Autio; Sami Kilpinen; Matti Saarela; Ilmo Leivo; Reidar Grénman; Antti A. Mäkitie; Outi Monni
• Keywords: squamous cell carcinoma; Gene Expression Profiling; Gene Amplification; Gene Dosage; Nucleic Acid Hybridization; cell lines; Neoplasm Proteins; Tongue Neoplasms; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; 0302 clinical medicine; SDG 3 - Good Health and Well-being; Cell Line, Tumor; gene expression; Carcinoma, Squamous Cell; Humans; head and neck cancer; microarray; Laryngeal Neoplasms; Gene Deletion; Oligonucleotide Array Sequence Analysis

Gene amplifications and deletions are frequent in head neck squamous cell carcinomas (SCC) but the association of these alterations with gene expression is mostly unknown. Here, we characterized genome-wide copy number changes on microarrays for 18 oral tongue SCC (OTSCC) lines. We identified a altered regions including nine high-level such as 6q12-q14 (CD109, MYO6), 9p24 (JAK2, CD274, SLC1A1, RLN1), 11p12-p13 (TRAF6, COMMD9, TRIM44, FJX1, CD44, PDHX, APIP), 11q13 (FADD, PPFIA1, CTTN), 14q24 (ABCD4, HBLD1, LTBP2, ZNF410, COQ6, ACYP1, JDP2) where 9% to 64% genes showed overexpression. Across whole genome, 26% amplified had associated overexpression OTSCC. Furthermore, our data implicated that OTSCC lines harbored similar genomic laryngeal have previously analyzed, suggesting despite differences clinicopathological features there no marked molecular genetic two HNSCC sites. To identify whose was increase SCC, statistical analysis line were performed. pinpointed 1,192 statistically significant between expression. These results suggest play an important role malignant behavior SCC. The provide basis further functional validation may lead identification novel candidates targeted therapies. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat.