Bleomycin‐induced chromosomal damage and shortening of telomeres in peripheral blood lymphocytes of incident cancer patients

• DOI: 10.1002/gcc.22508
• Publication Date: 2017-10-20T07:22:40Z
• Authors: Michal Kroupa; Zdenka Polivkova; Sivaramakrishna R...; Michaela Schneide...; Sona Vodenkova; Tomas Buchler; Katerina Jiraskova; Marketa Urbanova; Ludmila Vodickova; Kari Hemminki; Rajiv Kumar; Pavel Vodicka
• Keywords: Adult; Aged, 80 and over; Chromosome Aberrations; Male; 0301 basic medicine; 0303 health sciences; DNA Repair; Breast Neoplasms; Chromosome Disorders; Pilot Projects; Middle Aged; Telomere; Chromosomes; 3. Good health; Bleomycin; 03 medical and health sciences; Humans; DNA Breaks, Double-Stranded; Female; Lymphocytes; Colorectal Neoplasms; Aged

Disruption of genomic integrity due to deficient DNA repair capacity and telomere shortening constitute hallmarks malignant diseases. Incomplete or double-strand breaks (DSB) is manifested by chromosomal aberrations their frequency reflects inter-individual differences response exposure mutagenic compounds. In this study, we investigated in peripheral blood lymphocytes (PBL) from newly diagnosed cancer patients, including 47 breast (BC) 44 colorectal (CRC) patients 90 matched healthy controls. Mutagen sensitivity was evaluated measuring chromatid (CTAs) induced bleomycin supplemented the chemiluminescent measurement γ-H2AX phosphorylation 19 (11 BC, 8 CRC). Relative length (RTL) determined 22 32 CRC, 64 We observed statistically significant increased level CTAs (P = .03) percentage aberrant cells (ACs) with .05) CRC compared controls after treatment. No were between BC cases corresponding shorter RTL (below median) exhibited significantly higher amount ACs .02), high (≥12 CTAs/PBL; P such associations treatment PBL did not differ patients. Our results suggest that altered DSB measured towards mutagen occurs particularly carcinogenesis. Irrespective type, may be associated a decreased DSB.