Bleomycin‐induced chromosomal damage and shortening of telomeres in peripheral blood lymphocytes of incident cancer patients
Adult
Aged, 80 and over
Chromosome Aberrations
Male
0301 basic medicine
0303 health sciences
DNA Repair
Breast Neoplasms
Chromosome Disorders
Pilot Projects
Middle Aged
Telomere
Chromosomes
3. Good health
Bleomycin
03 medical and health sciences
Humans
DNA Breaks, Double-Stranded
Female
Lymphocytes
Colorectal Neoplasms
Aged
DOI:
10.1002/gcc.22508
Publication Date:
2017-10-20T07:22:40Z
AUTHORS (12)
ABSTRACT
Disruption of genomic integrity due to deficient DNA repair capacity and telomere shortening constitute hallmarks malignant diseases. Incomplete or double-strand breaks (DSB) is manifested by chromosomal aberrations their frequency reflects inter-individual differences response exposure mutagenic compounds. In this study, we investigated in peripheral blood lymphocytes (PBL) from newly diagnosed cancer patients, including 47 breast (BC) 44 colorectal (CRC) patients 90 matched healthy controls. Mutagen sensitivity was evaluated measuring chromatid (CTAs) induced bleomycin supplemented the chemiluminescent measurement γ-H2AX phosphorylation 19 (11 BC, 8 CRC). Relative length (RTL) determined 22 32 CRC, 64 We observed statistically significant increased level CTAs (P = .03) percentage aberrant cells (ACs) with .05) CRC compared controls after treatment. No were between BC cases corresponding shorter RTL (below median) exhibited significantly higher amount ACs .02), high (≥12 CTAs/PBL; P such associations treatment PBL did not differ patients. Our results suggest that altered DSB measured towards mutagen occurs particularly carcinogenesis. Irrespective type, may be associated a decreased DSB.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (56)
CITATIONS (12)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....