Enzymatic Laser‐Induced Graphene Biosensor for Electrochemical Sensing of the Herbicide Glyphosate

• DOI: 10.1002/gch2.202200057
• Publication Date: 2022-07-27T02:28:52Z
• Authors: Zachary T. Johnson; Nathan Jared; John K. Peterson; Jingzhe Li; Emily A. Smith; Scott A. Walper; Shelby L. Hooe; Joyce C. Breger; Igor L. Medintz; Carmen Gomes; Jonathan C. Claussen
• Keywords: Technology; T; 02 engineering and technology; 540; biosensors; glycine oxidase; 6. Clean water; Environmental sciences; herbicides; laser‐induced graphene; glyphosate; glyphosate ; herbicides ; glycine oxidase ; biosensors ; laser‐induced graphene; GE1-350; 0210 nano-technology; Research Articles

Abstract Glyphosate is a globally applied herbicide yet it has been relatively undetectable in‐field samples outside of gold‐standard techniques. Its presumed nontoxicity toward humans contested by the International Agency for Research on Cancer, while detected in farmers’ urine, surface waters and crop residues. Rapid, on‐site detection glyphosate hindered lack field‐deployable easy‐to‐use sensors that circumvent sample transportation to limited laboratories possess equipment needed detection. Herein, flavoenzyme, glycine oxidase, immobilized platinum‐decorated laser‐induced graphene (LIG) used selective as substrate GlyOx. The LIG platform provides scaffold enzyme attachment maintaining electronic properties graphene. sensor exhibits linear range 10–260 µ m , limit 3.03 sensitivity 0.991 nA −1 . shows minimal interference from commonly herbicides insecticides: atrazine, 2,4‐dichlorophenoxyacetic acid, dicamba, parathion‐methyl, paraoxon‐methyl, malathion, chlorpyrifos, thiamethoxam, clothianidin, imidacloprid. Sensor function further tested complex river water residue fluids, which validate this scalable, direct‐write, method mapping food analysis.