Dietary fat exacerbates postprandial hypothalamic inflammation involving glial fibrillary acidic protein‐positive cells and microglia in male mice

• DOI: 10.1002/glia.23882
• Publication Date: 2020-07-13T17:53:09Z
• Authors: Céline Cansell; Katharina Stobbe; Clara Sanchez; Ophélia Le Thuc; Coralie‐Anne Mosser; Selma Ben‐Fradj; Joris Leredde; Cynthia Lebeaupin; Delphine Debayle; Lucile Fleuriot; Frédéric Brau; Nadège Devaux; Alexandre Benani; Etienne Audinat; Nicolas Blondeau; Jean‐Louis Nahon; Carole Rovère
• Keywords: Astrogliosis; Neuroglia; Gliosis

In humans, obesity is associated with brain inflammation, glial reactivity, and immune cells infiltration. Studies in rodents have shown that reactivity occurs within 24 hr of high-fat diet (HFD) consumption, long before development, takes place mainly the hypothalamus (HT), a crucial structure for controlling body weight. Here, we sought to characterize postprandial HT inflammatory response 1, 3, 6 exposure either standard (SD) or HFD. HFD increased gene expression astrocyte microglial markers (glial fibrillary acidic protein [GFAP] Iba1, respectively) compared SD-treated mice induced morphological modifications HT. This remodeling was higher genes differential regulation hypothalamic neuropeptides involved energy balance regulation. DREADD PLX5622 technologies, used modulate GFAP-positive activity, respectively, showed both cell types are their own specific kinetics reactiveness ingested foods. Thus, recurrent exacerbated inflammation might promote needs be characterized address this worldwide crisis.