Microglia are involved in regulating histamine‐dependent and non‐dependent itch transmissions with distinguished signal pathways

• DOI: 10.1002/glia.24438
• Publication Date: 2023-07-01T08:34:39Z
• Authors: Yuxiu Yang; Bin Mou; Qi‐Ruo Zhang; Hong‐Xue Zhao; Jian‐Yun Zhang; Xiao Yun; Ming‐Tao Xiong; Ying Liu; Yong U. Liu; Haili Pan; Chao‐Lin Ma; Bao‐Ming Li; Jiyun Peng
• Keywords: CX3CR1

Although itch and pain have many similarities, they are completely different in perceptual experience behavioral response. In recent years, we a deep understanding of the neural pathways sensation transmission. However, there few reports on role non-neuronal cells itch. Microglia known to play key chronic neuropathic acute inflammatory pain. It is still unknown whether microglia also involved regulating transmission sensation. present study, used several kinds transgenic mice specifically deplete CX3CR1+ peripheral macrophages together (whole depletion), or selectively alone (central depletion). We observed that responses histamine, compound 48/80 chloroquine were all significantly reduced with either whole central depletion. Spinal c-fos mRNA assay further studies revealed histamine 48/80, but not elicited primary signal from DRG spinal Npr1- somatostatin-positive neurons relied microglial CX3CL1-CX3CR1 pathway. Our results suggested multiple types chemical transmission, while underlying mechanisms for histamine-dependent non-dependent former required