Mir-17-5P Promotes Migration of Human Hepatocellular Carcinoma Cells Through the P38 Mitogen-Activated Protein Kinase-Heat Shock Protein 27 Pathway

• DOI: 10.1002/hep.23566
• Publication Date: 2010-02-01T17:06:51Z
• Authors: Fu Yang; Yixuan Yin; Fang Wang; Yuqi Wang; Ling Zhang; Ying Tang; Shuhan Sun
• Keywords: Hsp27

miR-17-5p is overexpressed in hepatocellular carcinoma (HCC), but the specific regulatory mechanisms of HCC remain unknown. We investigated molecular basis as an oncogene human cell lines. Our vivo and vitro data indicate that up-regulates migration proliferation cells. Interestingly, proteomic western blotting assays revealed significantly activates p38 mitogen-activated protein kinase MAPK pathway increases phosphorylation heat shock 27 (HSP27). results also suggest E2F1-dependent down-regulation Wip1 regulates miR-17-5p-p38-HSP27 signaling. Furthermore, suppression HSP27 expression by small interfering RNA or pathway-specific inhibitor SB203580 decreases cells overexpressing does not reduce their proliferation. Finally, we show correlates well with status primary tissues metastasis. Conclusion: findings plays a crucial role miR-17-5p-induced and, consequence, phosphorylated enhances highlight important support potential application therapy. (Hepatology 2010;)