MicroRNA-125b Suppressesed Human Liver Cancer Cell Proliferation and Metastasis by Directly Targeting Oncogene LIN28B

• DOI: 10.1002/hep.23904
• Publication Date: 2010-08-20T03:18:23Z
• Authors: Linhui Liang; Chun-Ming Wong; Qiao Ying; Dorothy Ngo-Yin Fan; Shenglin Huang; Jie Ding; Jian Yao; Mingxia Yan; Jinjun Li; Ming Yao; Irene Oi-Lin Ng; Xianghuo He
• Keywords: Cell Division - drug effects; 0301 basic medicine; Down-Regulation; Transfection; Polymerase Chain Reaction; Colony-Forming Units Assay; 03 medical and health sciences; Cell Movement; Genes, Reporter; Cell Cycle - drug effects; Humans; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Cell Cycle; Liver Neoplasms; 500; RNA-Binding Proteins; MicroRNAs - genetics - pharmacology; Flow Cytometry; DNA-Binding Proteins - antagonists and inhibitors - drug effects; 3. Good health; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; MicroRNAs; Liver Neoplasms - genetics - pathology; Cell Division

MicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms hepatic carcinogenesis still unknown. Here we demonstrate suppressed HCC cell growth vitro vivo . Moreover, increased p21Cip1/Waf1 expression arrested cycle at G1 to S transition. In addition, inhibited migration invasion. Further studies revealed LIN28B downstream target of cells bound directly the 3′ untranslated region , thus reducing both messenger RNA protein levels Silencing recapitulated effects overexpression, whereas enforced reversed suppressive miR-125b. Conclusion: These findings indicate exerts tumor-suppressive through suppression oncogene suggest therapeutic application HCC. (Hepatology 2010)