MicroRNA-140-5p suppresses tumor growth and metastasis by targeting transforming growth factor β receptor 1 and fibroblast growth factor 9 in hepatocellular carcinoma

• DOI: 10.1002/hep.26315
• Publication Date: 2013-02-11T09:58:52Z
• Authors: Hao Yang; Feng Fang; Ruimin Chang; Lianyue Yang
• Keywords: Ectopic expression; FGF9

By comparing the expression profiles of microRNAs (miRNAs) in different hepatocellular carcinoma (HCC) subtypes, we identified miR-140-5p as an HCC-related miRNA. We found that was significantly decreased HCC tissues and all six liver cancer cell lines examined its levels were correlated with multiple nodules, vein invasion, capsular formation, differentiation, well overall disease-free survival HCC. also suppressed proliferation metastasis. Multipathway reporter arrays suggested inhibited transforming growth factor β (TGF-β) mitogen-activated protein kinase / extracellular signal-regulated (MAPK/ERK) signaling. TGFB receptor 1 (TGFBR1) fibroblast 9 (FGF9) then characterized direct targets for after it ectopic TGFBR1 FGF9 expression. Silencing by small interfering RNA (siRNA) resembled phenotype resulting from expression, while overexpression attenuated effect on metastasis.These data elucidated a tumor suppressor role development progression therapeutic potential. Our correlation studies clinical samples further suggest could be valuable biomarker prognosis.