In vitro and in vivo anti‐tumoral effect of curcumin against melanoma cells

• DOI: 10.1002/ijc.20160
• Publication Date: 2004-04-23T16:53:20Z
• Authors: Johann Odot; Philippe Albert; Annie Carlier; Michel Tarpin; Jérôme Devy; Claudie Madoulet
• Keywords: 0301 basic medicine; Curcumin; Melanoma, Experimental; Antineoplastic Agents; Apoptosis; DNA, Neoplasm; 3. Good health; Mice; 03 medical and health sciences; Cell Line, Tumor; Animals; Female; Cell Division; Phytotherapy

Curcumin, the active ingredient from spice turmeric (Curcuma longa Linn), is known to be an anti-oxidant and anti-inflammatory agent. It has been demonstrated recently possess anti-angiogenic effects pro-apoptotic activities against Ehrlich ascites tumor cells. In current study, curcumin was found cytotoxic in vitro for B16-R melanoma cells resistant doxorubicin either cultivated as monolayers or grown three-dimensional (3-D) cultures (spheroids). We have that effect observed 2 culture types can related induction of programmed cell death. our vivo studies, we examined effectiveness a prophylactic immune preparation soluble proteins cells, treatment with soon tumoral appearance, alone combination, on murine B16-R. The combination resulted substantial inhibition growth melanoma, whereas each by itself showed little effect. Moreover, animals receiving therapy exhibited enhancement their humoral anti-soluble protein response significant increase median survival time (> 82.8% vs. 48.6% 45.7% respectively immunized group curcumin-treated group). Our study shows may provide valuable tool development therapeutic melanoma.