iNOS‐dependent DNA damage via NF‐κB expression in hamsters infected with Opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel

• DOI: 10.1002/ijc.21893
• Publication Date: 2006-03-28T21:19:01Z
• Authors: Somchai Pinlaor; Yusuke Hiraku; Puangrat Yongvanit; Saeko Tada‐Oikawa; Ning Ma; Porntip Pinlaor; Paiboon Sithithaworn; Banchob Sripa; Mariko Murata; Shinji Oikawa; Shosuke Kawanishi
• Keywords: Opisthorchis viverrini

Inflammation-mediated DNA damage triggered by Opisthorchis viverrini (OV) infection is a major risk factor of cholangiocarcinoma (CCA). We have recently reported that nitrative and oxidative participates in CCA development caused repeated with OV [Pinlaor et al., Carcinogenesis 2004; 25:1535-42]. Therefore, to clarify the preventive effect antihelminthic drug praziquantel against cholangiocarcinogenesis, we assessed this on damage, including formation 8-nitroguanine 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), expression inducible nitric oxide synthase (iNOS) immunohistochemistry OV-infected hamsters. also examined nuclear factor-kappaB (NF-kappaB), which functions as tumor promoter inflammation-associated cancer. Our results showed although 1-week treatment did not kill parasites completely hamsters days 14 30, dramatically reduced inflammatory cell infiltration. Double immunofluorescence staining almost diminished OV-induced 8-oxodG bile duct epithelial cells. Quantitative analysis using an electrochemical detector coupled HPLC revealed level liver was significantly decreased (p<0.05). Western blotting NF-kappaB iNOS epithelium treatment. The amount nitrate plus nitrite plasma after It concluded can exhibit inhibiting iNOS-dependent through only elimination but potential antiinflammatory effect.