MicroRNA‐196a promotes an oncogenic effect in head and neck cancer cells by suppressing annexin A1 and enhancing radioresistance

• DOI: 10.1002/ijc.29397
• Publication Date: 2014-12-19T11:28:38Z
• Authors: Yae‐Eun Suh; Nina Raulf; Joop Gäken; Katherine Lawler; Teresa Guerrero U...; Jessica Bullenkamp; Stéphane Gobeil; Jacques Huot; Eddy Odell; Mahvash Tavassoli
• Keywords: Radioresistance

Radiotherapy is a major treatment modality for head and neck squamous cell carcinoma (HNSCC). Up to 50% of patients with locally advanced disease relapse after radical there therefore need develop predictive bomarkers clinical use that allow the selection who are likely respond. MicroRNA (miRNA) expression profiling panel HNSCC tumours without recurrent surgery radiotherapy detected miR‐196a as one highest upregulated miRNAs in poor prognostic group. To further study role miR‐196a, its was determined eight cancer lines. Overexpression cells, low endogenous expression, significantly increased proliferation, migration invasion, induced epithelial mesenchymal transition. Conversely, knockdown cells high levels reduced oncogenic behaviour. Importantly, overexpression radioresistance measured by gamma H2AX staining MTT survival assay. Annexin A1 (ANXA1), known target found be directly modulated luciferase assay confirmed Western blot analysis. ANXA1 exhibited similar phenotypic effects overexpression, suggesting effect may at least partly regulated through suppression ANXA1. In conclusion, this identifies potential important biomarker prognosis response radiotherapy. Furthermore, our data suggest and/or gene could represent therapeutic targets HNSCC.