Rapid differentiating colorectal cancer and colorectal polyp using dried blood spot mass spectrometry metabolomic approach

• DOI: 10.1002/iub.1617
• Publication Date: 2017-03-21T03:28:47Z
• Authors: Yu Jing; Xue Wu; Peng Gao; Zhongze Fang; Jingjing Wu; Qingjun Wang; Chen Li; Zhitu Zhu; Yunfeng Cao
• Keywords: Colorectal Polyp

Colorectal cancer (CRC) is the third leading causes of mortality, and early-stage detection could significantly enhance survival rates. Cancer influences important metabolic pathways changes in metabolite levels had been used many studies as potential biomarkers. This study aimed at screening biomarkers with CRC diagnosis potentials. The direct infusion mass spectrometry (MS) metabolomic analysis based on dried blood spot was to distinguish from polyp. target metabolites were composed 23 amino acids 26 acylcarnitines. 21 selected via multivariate analysis. A regression model established parameters C16, Arg, C4/C8, C5/C3, Val, Phe/Tyr, Ala, C4/C3. Tenfold cross validation (CV) method test this showed sensitivity 81.18% specificity 83.95%. a practicable for detection. use MS be finished several minutes served higher-throughput polyps. © 2017 IUBMB Life, 69(5):347-354, 2017.