Purified c‐phycoerythrin: safety studies in rats and protective role against permanganate‐mediated fibroblast‐DNA damage

• DOI: 10.1002/jat.1524
• Publication Date: 2010-04-30T01:37:37Z
• Authors: Badrish Soni; Nishant P. Visava...; Nirav Dalwadi; Datta Madamwar; Chris Winder; Christian Khalil
• Keywords: Phycoerythrin; Comet Assay; Potassium permanganate; Neutral red

Abstract We have evaluated in vitro cytotoxicity of cyanobacterial phycoerythrin (C‐PE) on three human cell lines by proliferation and neutral red uptake assays. No toxic effects C‐PE were observed to any the tested. The protective role purified potassium permanganate‐mediated fibroblast‐DNA damage was assessed comet assay at 0 (control), 10 20 µg ml −1 doses pre‐, simultaneous post‐mutagen exposure conditions. Significant DNA detected only Our findings confirmed that is non‐toxic provides protection against damage. preliminary acute (2000 mg kg body weight, b.w.) 90 day sub‐chronic (0, 5, 15 25 b.w./day) oral toxicity studies male albino rats showed no mortality or treatment‐related major clinical signs, all well tolerated. adverse effect level found be 5 b.w./day respectively. Copyright © 2010 John Wiley & Sons, Ltd.