Raman and FTIR microscopic imaging of colon tissue: a comparative study

• DOI: 10.1002/jbio.200710005
• Publication Date: 2008-02-05T09:46:30Z
• Authors: Christoph Krafft; Daniela Codrich; Gloria Pelizzo; Valter Sergo
• Keywords: Raman microscope; Infrared microscopy; Circular muscle

Colon tissue constitutes a valid model for the comparative analysis of soft by Raman and Fourier transform infrared (FTIR) imaging because it contains four major types such as muscle tissue, connective epithelium nerve cells. microscopic images were recorded in mapping mode using 785 nm laser excitation step size 10 microm from three regions within thin section that encompassed mucus, mucosa, submucosa, longitudinal circular layers. FTIR composed 4, 8 9 individual 4096 spectra each same spectrometer coupled to microscope with focal plane array detector. Furthermore, at 2.5 ganglia belong myenteric plexus. The results are discussed respect lateral resolution, spectral acquisition time sensitivity both modalities.