Label‐free imaging and analysis of the effects of lipolysis products on primary hepatocytes

• DOI: 10.1002/jbio.201000086
• Publication Date: 2010-09-28T06:23:23Z
• Authors: Iwan W. Schie; Jian Wu; Tyler Weeks; Mark A. Zern; John C. Rutledge; Thomas Huser
• Keywords: Lipid droplet; Degree of unsaturation

Abstract The increased accumulation of intracellular lipid droplets within hepatocytes is a pathologic hallmark liver injury various etiologies, especially non‐alcoholic steatohepatitis (NASH). dynamics, subcellular origin, and chemical composition under pathophysiologic conditions, however, remain poorly understood. We used coherent Raman microscopy spontaneous spectroscopy to monitor analyze the formation in living primary rat exposed triglyceride‐rich lipoprotein (TGRL) lipolysis products. After exposure complex fatty acid mixture released during process for 30 minutes, new rapidly appeared size number over total observation period 205 minutes. spectroscopic analysis individual before after products reveals that major components these are esterified unsaturated acids. find unsaturation ratio increases with droplet size. Control experiments defined mixtures reveal complexity cellular response assault by combinations lipids. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)