In vivo deep brain multiphoton fluorescence imaging emitting at NIR‐I and NIR‐II and excited at NIR‐IV
0301 basic medicine
Mice
0303 health sciences
Microscopy, Fluorescence, Multiphoton
Spectroscopy, Near-Infrared
Optical Imaging
Animals
Brain
Fluorescent Dyes
DOI:
10.1002/jbio.202300422
Publication Date:
2024-01-12T01:14:35Z
AUTHORS (12)
ABSTRACT
Abstract Multiphoton microscopy (MPM) enables deep brain imaging. Three optical windows: NIR‐I, NIR‐II, and NIR‐III are widely used. Recently, NIR‐IV (the 2200 nm window) has been demonstrated to be the last longest window for tissue MPM. However, so far MPM covers only two windows labeled by single fluorescent probe, one emission excitation. Here we demonstrate in vivo covering three windows, with at excitation NIR‐IV, ICG. The innovations include: (1) characterizing both 3‐photon properties of ICG emitting NIR‐I water, plasma, circulating blood; (2) a home‐built multiphoton microscope simultaneous dual channel detection, which 950 μm (NIR‐I) 850 (NIR‐II) into mouse vivo, verifying that multi‐optical is promising
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (32)
CITATIONS (3)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....