In vivo deep brain multiphoton fluorescence imaging emitting at NIR‐I and NIR‐II and excited at NIR‐IV

• DOI: 10.1002/jbio.202300422
• Publication Date: 2024-01-12T01:14:35Z
• Authors: Jincheng Zhong; Yingxian Zhang; Xinlin Chen; Shen Tong; Xiangquan Deng; Jie Huang; Zhenhui Li; Chi Zhang; Zhiang Gao; Jia Li; Ping Qiu; Ke Wang
• Keywords: 0301 basic medicine; Mice; 0303 health sciences; Microscopy, Fluorescence, Multiphoton; Spectroscopy, Near-Infrared; Optical Imaging; Animals; Brain; Fluorescent Dyes

Abstract Multiphoton microscopy (MPM) enables deep brain imaging. Three optical windows: NIR‐I, NIR‐II, and NIR‐III are widely used. Recently, NIR‐IV (the 2200 nm window) has been demonstrated to be the last longest window for tissue MPM. However, so far MPM covers only two windows labeled by single fluorescent probe, one emission excitation. Here we demonstrate in vivo covering three windows, with at excitation NIR‐IV, ICG. The innovations include: (1) characterizing both 3‐photon properties of ICG emitting NIR‐I water, plasma, circulating blood; (2) a home‐built multiphoton microscope simultaneous dual channel detection, which 950 μm (NIR‐I) 850 (NIR‐II) into mouse vivo, verifying that multi‐optical is promising