Attachment and spreading of fibroblasts on an RGD peptide–modified injectable hyaluronan hydrogel

• DOI: 10.1002/jbm.a.20002
• Publication Date: 2003-12-29T22:00:39Z
• Authors: Xiao Zheng Shu; Kaustabh Ghosh; Yanchun Liu; Fabio S. Palumbo; Yi Luo; Richard A. Clark; Glenn D. Prestwich
• Keywords: 0301 basic medicine; Time Factors; Cell Culture Techniques; RGD, Hyaluronic acid; Biocompatible Materials; Hydrogels; Fibroblasts; Mice; 03 medical and health sciences; Cell Movement; Cell Adhesion; NIH 3T3 Cells; Animals; Hyaluronic Acid; Oligopeptides

Hyaluronan (HA) hydrogels resist attachment and spreading of fibroblasts most other mammalian cell types. A thiol-modified HA (3,3'-dithiobis(propanoic dihydrazide) [HA-DTPH]) was modified with peptides containing the Arg-Gly-Asp (RGD) sequence then crosslinked polyethylene glycol (PEG) diacrylate (PEGDA) to create a biomaterial that supported attachment, spreading, proliferation. The were evaluated in vitro vivo three assay systems. First, behavior human murine on surface evaluated. concentration structure RGD length PEG spacer influenced spreading. Second, seeded into HA-DTPH solutions encapsulated via situ crosslinking or without bound peptides. Cells remained viable proliferated within hydrogel for 15 days vitro. Although significantly enhanced proliferation surface, inside increased only modestly. Third, HA-DTPH/PEGDA/peptide as injectable tissue engineering materials vivo. suspension using PEGDA plus peptide, viscous, gelling mixture injected subcutaneously flanks nude mice; gels formed following injection. After 4 weeks, growth new fibrous had been accelerated by sense Thus, cells is dramatically RGD-modified surfaces but modestly formation.